![A high-efficiency method for site-directed mutagenesis of large plasmids based on large DNA fragment amplification and recombinational ligation | Scientific Reports A high-efficiency method for site-directed mutagenesis of large plasmids based on large DNA fragment amplification and recombinational ligation | Scientific Reports](https://media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41598-021-89884-z/MediaObjects/41598_2021_89884_Fig1_HTML.png)
A high-efficiency method for site-directed mutagenesis of large plasmids based on large DNA fragment amplification and recombinational ligation | Scientific Reports
![Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies | BMC Biotechnology | Full Text Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies | BMC Biotechnology | Full Text](https://media.springernature.com/lw685/springer-static/image/art%3A10.1186%2F1472-6750-9-61/MediaObjects/12896_2009_Article_427_Fig1_HTML.jpg)
Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies | BMC Biotechnology | Full Text
![A simple and economical site-directed mutagenesis method for large plasmids by direct transformation of two overlapping PCR fragments | BioTechniques A simple and economical site-directed mutagenesis method for large plasmids by direct transformation of two overlapping PCR fragments | BioTechniques](https://www.future-science.com/cms/10.2144/btn-2022-0085/asset/images/medium/figure1.gif)
A simple and economical site-directed mutagenesis method for large plasmids by direct transformation of two overlapping PCR fragments | BioTechniques
![Schematic diagram of the primer design for site-directed mutagenesis.... | Download Scientific Diagram Schematic diagram of the primer design for site-directed mutagenesis.... | Download Scientific Diagram](https://www.researchgate.net/profile/James-Naismith/publication/23570761/figure/fig2/AS:196065824382979@1423757143246/Schematic-diagram-of-the-primer-design-for-site-directed-mutagenesis-Primer-designs-are_Q640.jpg)
Schematic diagram of the primer design for site-directed mutagenesis.... | Download Scientific Diagram
![New England Biolabs (UK) Ltd - Q5<sup>®</sup> Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs (UK) Ltd - Q5<sup>®</sup> Site-Directed Mutagenesis Kit (Without Competent Cells)](https://international.neb.com/-/media/catalog/long-description/e0552b.jpg?rev=f167057cb3ab45d9ba0814f4988ed512&hash=C68FD638A2885377C206C0B361A4561D)
New England Biolabs (UK) Ltd - Q5<sup>®</sup> Site-Directed Mutagenesis Kit (Without Competent Cells)
![Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy | SpringerLink Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy | SpringerLink](https://media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00253-018-9041-2/MediaObjects/253_2018_9041_Fig1_HTML.png)
Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy | SpringerLink
![Enhanced Mutant Screening in One-step PCR-based Multiple Site-directed Plasmid Mutagenesis by Introduction of Silent Restriction Sites for Structural and Functional Study of Proteins | Biological Procedures Online | Full Text Enhanced Mutant Screening in One-step PCR-based Multiple Site-directed Plasmid Mutagenesis by Introduction of Silent Restriction Sites for Structural and Functional Study of Proteins | Biological Procedures Online | Full Text](https://media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs12575-017-0062-5/MediaObjects/12575_2017_62_Fig1_HTML.gif)